################################################################################################# # BoostDDG: Accurately predicting stability changes upon point mutations from protein sequences # ################################################################################################# # This file describes the input format for the BoostDDG web-server. # Input files should not contain suffixes. # Each mutation to be predicted contains two lines, the first line should start with 'SEQ:' and the second line should start with 'MUT:'. # 'SEQ:' line defines the target protein sequence in a one-letter code: 'SEQ: proteinId protein_sequence'. # 'MUT:' line defines the mutations which are to be predicted for the protein sequence specified on the previous line: 'MUT: XnY ...'. # proteinId: protein sequence unique identifier (the identifier should not contain '.'). # mutation: The mutation format 'XnY' stands for X: wild-type amino acid, n: 1-based sequence position, Y: mutated amino acid. # Multiple mutations per line are allowed, however, they are always predicted as single-site mutations at a time. # Please note that unknown amino acids such as 'X' should be removed beforehand. # The whole protein sequence must be specified on a single line, i.e., line-breaks are not allowed. ############################################################################ # Please note that the input size is limited to one proteins per submission # ############################################################################ ############ # Examples # ############ # Example of a query with three mutations (each mutation is predicted as a single-site mutation): SEQ: 1W3D GSAEAEAGPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRVLKEDIDAFLAGGA MUT: A5G A52G A52D #carry out all possible substitutions for the given position 'n' of amino acid 'X': 'Xn*' SEQ: 1W3D GSAEAEAGPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRVLKEDIDAFLAGGA MUT: A52*